Resources

PLASMIDS

We receive very large numbers of requests for plasmid materials every month. Fulfilling the volume of requests is beyond our capacity. However, we’re very keen to see our research help your research. To enable this, we deposit all our plasmids at Addgene. You can find all of our published plasmids at https://www.addgene.org/Nicola_Patron/. Addgene stores, sequences and distributes plasmids on behalf of thousands of labs, helping scientists make research materials available to the community on a non-profit, cost-recovery basis (requestors pay a fee to cover shipping, handling, maintenance and quality assurance). Addgene has a streamlined Material Transfer Agreement (MTA) process and stays up-to-date with the ever-changing requirements of how to ship biomaterials to most countries. If you deposit your own plasmids, you will accrue points that allow you to request plasmids free of charge.

PROTOCOLS

We use a Type IIS parallel DNA assembly method (also known as Golden Gate Cloning). We have published a toolkit for plants as well as the PhytoBrick TypeIIS standard. Full details and a  laboratory protocol can be found in this methods chapter: Patron, NJ (2016). DNA assembly for plant biology. Current Protocols in Plant Biology 1:604-616.  You can also download a help-sheet on how to make new parts.

CODE

## CRISPRanto“; a (Snakemake) pipeline for CRISPR offtarget detection used in Raitskin et al (2019)